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SKU
MN/740966.50
Brand
MACHEREY-NAGEL
Pre-order (Deliver in 8 to 12 weeks)
NucleoSpin TriPrep (50)
Parallel isolation of RNA, DNA and, protein from an undivided sample
| Application | Isolation of RNA, DNA, and protein |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Animal tissue, Cells, Human tissue, Plant |
| Sample amount | < 5 x 106 cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue |
| Fragment size | RNA > 200 nt, DNA < 30 kbp, protein 15–300 kDa |
| Typical yield | < 70 µg RNA, < 6 µg DNA, < 1200 µg Protein |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | RNA 1.9-2.1, DNA 1.7-1.9 |
| Typical RIN | > 9 |
| Elution volume | RNA 40–120 μL , DNA 100 μL , Protein 10–100 μL |
| Preparation time | 30 min/6 preps (RNA), 45 min/6 preps (RNA and DNA), + 35 min/6 preps (protein) |
| Typical downstream application | Chip hybridization, qRT-PCR, RNA-Seq |
| Storage temperature | 18-25 °C |
| Shelf life (from production) | 24 Months |
| Hazardous material | Yes |
| Brand | MACHEREY-NAGEL |
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