MIDDLEBROOK BROTH 500 grams/bottle

Middlebrook Broth, complemented with various supplements, is used to isolate a wide variety of Mycobacteria, including M. tuberculosis, but with the exception of M. bovis, which is inhibited by glycerol. Inorganic salts provide substances essential for the growth of Mycobacteria. Sodium citrate, when converted to citric acid, serves to hold certain inorganic cations in solution. Albumin neutralizes toxic products that form during the development of the organisms. Catalase catalyzes the decomposition of hydrogen peroxide to water and oxygen. Dextrose is is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Supplementation with glycerol enhances the growth of mycobacteria.