His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of anÂ
Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
- Robust parallel screening with high reproducibility
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Capture histidine-tagged protein from different sample volumes (low microliter to high milliliter scale)
- Visible and dense Sepharose based magnetic beads give increased simplicity for handling
- High density of beads ensures rapid capture by magnetic devices
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells inÂ
HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Compatible withÂ
MagRack 6Â andÂ
MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Validate your login