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Calcium Caseinate Agar is used as a selective medium for the recovery of proteolytic microorganisms in foods. The industrial food processes cause sublethal injuries to many microorganisms and, to aid in recovery, nutrient-rich media are used. This medium contains casein, a raw milk source rich in amino acids and nitrogen which is degraded by the proteolytes to form clearer zones surrounding the colonies in an otherwise turbid medium. Meat peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Calcium hydroxide is the inhibitor. Calcium Chloride helps to maintain the pH of the medium. Bacteriological Agar is the solidifying agent.
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