Brilliant Green Agar Modified is a selective medium for the isolation of Salmonella, except for S.typhy, from water,food and animal feed stuffs. Brilliant Green Agar Modified inhibits the growth of Pseudomonas aeruginosa and partially inhibits the growth of Proteus spp. which may be similar in appearance to Salmonella. Salmonella can be present in small numbers and is often accompained by considerably larger numbers of other Enterobacteriaceae or bacteria of other families. A preenrichment stage is used to permit the detection of low numbers of Salmonella or injured Salmonella. Beef extract, Casein peptone and Meat peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Lactose and Sucrose are the fermentable carbohydrate providing carbon and energy. Phenol red is the pH indicato. Brilliant green inhibits Gram-positive and most Gram-negative bacteria, except Salmonella. If the medium overheats, brilliant green may lose its properties. Bacteriological agar is the solidifying agent. The ISO normative 6579 recommends the Brilliant Green Agar as a second selective medium.
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