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Uncategorized > Product SKU: GE/27036101

READY-TO-GO T4 DNA LIGASE 50 RXNS

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RM 5,873.00

Pre-order

Ready-To-Go T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments.

  • Covalently-linking DNA fragments takes only 30 minutes.
  • Single-dose, ambient temperature stable reaction mixes.

Ready-To-Go T4 DNA Ligase includes self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA.

Adenosine triphosphate (ATP) is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.