READY-TO-GO T4 DNA LIGASE 50 RXNS
Ready-To-Go T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments.
- Covalently-linking DNA fragments takes only 30 minutes.
- Single-dose, ambient temperature stable reaction mixes.
Ready-To-Go T4 DNA Ligase includes self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA.
Adenosine triphosphate (ATP) is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.