NucleoSpin miRNA (50)

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RM 1,757.00

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Parallel isolation of small and large RNA from various sample types

ApplicationIsolation of total RNA
TargetRNA
CE certifiedNo, research use only
TechnologySilica membrane technology
BrandNucleoSpin
FormatMini prep
Package unit10 Prep(s), 50 Prep(s), 250 Prep(s)
HandlingCentrifugation
Lysate clarificationCentrifugation, NucleoSpin Filter
Automated useNo
Sample materialAnimal tissue, Cells, Human tissue, Reaction mixture
Sample amount< 10⁷ cultured cells, < 30 mg human / animal tissue, < 50 mg plant tissue, < 150 µL reaction mixtures,
Fragment size> 18 nt
Typical yield10 µg small RNA, 90 µg large RNA (10⁷ HeLa cells)
Theoretical binding capacity200 µg
Elution volume30–100 µL
Preparation time45 min/6 preps (total RNA)
Typical downstream applicationArray technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq
Storage temperature18−25 °C
Shelf life (from production)24 Months
Hazardous materialYes
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