SKU: MN/740410.50

NucleoBond Xtra Midi (50)


RM 2,391.00


2nd generation of anion exchanger for fast purification of plasmid DNA

ApplicationTransfection-grade plasmid DNA isolation
TargetPlasmid DNA
CE certifiedNo, research use only
TechnologyAnion exchange chromatography
FormatMidi prep
Package unit10 Prep(s), 50 Prep(s), 100 Prep(s)
HandlingGravity flow
Lysate clarificationColumn filter
Automated useNo
Sample materialBacteria, E. coli
Sample amount< 200 mL (high copy), < 400 mL (low copy)
Vector size< 300 kbp
Typical yield500 µg
Theoretical binding capacity800 µg
Typical purity A260/A2801.8−1.95
Endotoxin level1−10 EU/µg DNA
Isopropanol precipitationCentrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time70 min/prep
Typical downstream applicationCloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature18−25 °C
Shelf life (from production)39 Months
Hazardous materialYes