RM 1,066.00
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Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
- Sample protection: Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
- High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
- Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
- Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 μL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.
- Download the Vivaspin protein concentrator data file to learn more and to determine the right MWCO (molecular weight cut-off) and volume for your enrichment study.
Spin column protein concentration – how does it work?
Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.