MICROSPIN G-50 COLUMNS, 50 COLUMNS
MicroSpin G-50 columns are designed for the rapid purification of DNA in desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions.
- For rapid purification of labeled DNA (DNA probes of > 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μL using spin-column chromatography.
- Columns are prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
- Product recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.
- Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μl) following heat denaturation and phenol extraction.
MicroSpin G-50 Columns, rapid purification of DNA requiring clean-up
Good product yield and purity is obtained with sample volumes from 12-50 μL. They are suitable for any DNA greater than 20 bases in length and will not remove or denature enzymes.
The resin used in these columns, Sephadex G-50 DNA Grade, can be used for a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.
Alternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.
Sephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.
All these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.