Endo Base Broth, 500G/Btl 500 grams/bottle (CDL/1276)
Endo Base Broth is used for the identification of Enterobacteriaceae
Peptone, tryptose and tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Phosphates act as a buffer system. Yeast extract is a source of vitamins, particularly of the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium desoxycholate inhibit growth of gram positive bacteria. Sodium lauryl sulphate partially inhibits organisms other than coliforms. The moderate selectivity is due to the formation of a fuchsine-sulfite compound. This compound reacts with the acetaldehyde formed in the lactose fermentation and frees the fuchsin dye that colours the bacterial colony.
This medium is normally used to impregnate absorbent materials (e.g. cardboard discs) on which the inoculated filters are placed. After filtration, the filters are placed on the soaked pieces of cardboard.